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Once you're enrolled. GFP green represents cells expressing the indicated plasmids; TU20 ab, red represents immature neurons. Confirmation of differentiation of iPSCs into cortical neuronal cultures.
Images taken from clones C2 trisomic and C3 -D21 disomic 40 days after initiation of the differentiation protocol. Fixed cells on chamber slides were probed with antibodies against the marker proteins listed in the left column. Negative controls: 1— Rabbit primary and anti-mouse secondary antibody; 2 — Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
The gel was run at V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer pH 6, epitope retrieval solution 1 for 20 mins. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. No primary antibody was used in the secondary only control shown on the inset. For other IHC staining systems automated and non-automated customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Cells were fixed with paraformaldehyde, permeabilized with 0. Permeabilization: No. Floorplate region of developing cervical spinal cord I have not studied this particular region. To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you get on. Publishing research using ab? Please let us know so that we can cite the reference in this datasheet. Your name Your email. Send me a copy of this email. I agree to the terms and conditions. Related conjugates and formulations. Class III beta-tubulin specific for neurons.
Reacts with: Mouse, Human Predicted to work with: a wide range of other species, Mammals. It is reactive with human, mouse, rat and other species. IgM Monoclonal. Recognizes the N-terminal structural domain of Beta-Tubulin. Reacts with all isoforms of beta-tubulin. Species cross-reactivity: Human, mouse, rat, hamster, porcine, bovine, chicken, carp, plants, yeasts, protozoa.
Avoid freeze-thaw cycles. For research use only. Intended for use by laboratory professionals. Publications 0. Submit a Publication for LS-C Customer Reviews 0.
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